Productos ATTO
Propósito y Aplicación
-
Imágenes de quimioluminiscencia de transferencia Western
-
Imágenes fluorescentes de geles de tinción fluorescente y proteínas fluorescentes (opcional)
-
Imágenes de campo brillante de geles de tinción CBB (opcional)
-
Análisis cuantitativo de muestras a partir de la imagen (Concentración y Peso Molecular)
-
Combinar imagen de imagen quimioluminiscente y marcador preteñido de imagen de membrana
Características
-
El primer objetivo "F0.8" del mundo
Es 10% ~ 300% más brillante que otros fabricantes en el mercado y puede detectar luz débil.
ATTO no se basa en el procesamiento digital y ATTO actualizó un amplio rango dinámico con un rendimiento de baja distorsión para crear imágenes de alta calidad. -
Combinar imagen de imagen luminiscente e imagen de membrana
-
Función de exposición automática
→con rango dinámico que procesa la reducción de ruido (aritmética oscura, procesamiento de reducción de ruido), corrección de campo plano, etc. en automático -
Formato TIFF de 16 bits
→Formato de archivo de imagen general
→Convertible a JPEG y TIFF de 8 bits -
Imagen inversa de contraste automático
→El software de control y el software de análisis de imágenes están disponibles para la función de contraste automático e imagen inversa
WSE-6200 LuminoGraph II
Soluciones de transferencia Western
Sistema de documentación de geles
Sistema de imágenes de quimioluminiscencia
Software de análisis de imágenes
Referencia Fuente de luz LED
Investigación de células vivas
Centrifuse / Block Incubator / Otros
①CAMERA LENS FILTER
CAMERA : COOLING CMOS CAMERA
Pixels : 2688 x 1512 (4 Mpixels)
Lens : F0.95 single-focus lens
Filter (optional) : YA-3 (LPF560), ND-0.1 (diminished filter)
The high-sensitivity cooling CMOS camera enables high-sensitivity chemiluminescence samples.
If you use the sensitivity on gain function,
"HQ" → "STD" → "HIGH" → "ULTRA"
You can increase the detection sensitivity without compromising the resolution.
②White light source (Optional)
CBB-dyed gel, silver-dyed gel, etc., are used with a white transmission light source with less uniform.
It can be photographed with a high-resolution image of
4 megapixels.
Photographed images can be stored in 16-bit TIFF and are suitable for quantitative analysis.
③Cyan Transparent Light Source (Optional)
A transmission-type fluorescent irradiation device with a peak wavelength of 505 nm (half-width ± 25 nm) can detect various fluorescent staining reagents, including ethidium bromide.
Unlike binning, gain-based sensitivity adjustment can increase the sensitivity itself without compromising image resolution.
It shows the results of western blotting of transferrin proteins that in a 1/2 dilution sequence from 10ng/lane with anti-transferrin antibodies. It is a photographic image of luminescence after reaction with an HRP luminescence substrate.
[Experimental conditions]
Gel : M-520L
Sample : Human Transferrin
(1 ng left to 1/2 dilution sequence from lane)
Transcription Criteria: QBlot W, 24V, 15 mins
Blocking : EzBlockCAS, 30 mins
Antibodies:
Primary antibody: anti-human transferrin rabbit polyclonal antibody
Secondary antibody : HRP-labeled anti-rabbit Ig antibody
emission detection : EzWestLumi plus
Detection device : LuminoGraph I CMOS
Exposure time : 1 min
A sample of HeLa cell extraction protein added 0-5 ng of human albumin per lane was separated by u-PAGEL H and transcribed by QBlot Mini, and the total protein on the PVDF membrane was detected by EzStain AQua MEM.
Western blotting was performed using anti-human albumin antibodies and light emission was detected by EzWestLumi plus.
The image above shows the total protein (bright field) and target protein (luminescence) taken with LuminoGtaph I CMOS.
The graph is the result of analyzing each image data with CSanalyzer 4 and normalizing the amount of albumin based on the signal value of all proteins.
We were able to detect albumin in a wide concentration range of 3 orders, ranging from 7 pg to 5000 pg, with a signal intensity approximate to the theoretical value.
LuminoGraph I CMOS can detect low concentrations of weak luminescence with high sensitivity, and can be photographed in a wide dynamic range without saturation of high concentrations of protein.
image
[Experimental conditions]
Gel : UH-T420
Sample : Cell extract with human albumin added
HeLa cell extract (20μg/all lanes)
human albumin
(5 ng from left / 1/3 dilution sequence from lane)
Transcription Criteria : QBlot M, 24V, 15 mins
Blocking : EzBlockCAS, 30 mins
ANTIBODY: HRP-LABELED ANTI-HUMAN ALBUMIN
emission detection : EzWestLumi plus
Photographic equipment : LuminoGraph I CMOS
Bright field : Interior lighting, 10 ms
Luminescence : 10s
It shows the results of a 30-minute-stained gel Electrophoresis with a fluorescent reagent through a size marker of DNA.
[Experimental conditions]
Gel : EHR-T7.5L
Buffer : EzRun TG
Sample : Various DNA Molecular Weight Standards
Gel Dyeing : EzFluoroStain DNA
EzPreStain DNA & RNA
Photographic equipment : LuminoGraph I CMOS
filters : YA-3 (optional)
Light Source : CyanoView (optional)
Filming time : EzFluoroStain DNA 20 ms
EzPreStain DNA & RNA 50 ms
image of a CBB dyed (EzStain AQua) gel taken using a combination of displayed light sources and filters.
The CBB stain gel was subjected to pseudo-color treatment with CS Analyzer after detection.
[Experimental conditions]
Gel : P-R16.5S (trisin gel)
Buffers: EzRun T
Sample : Chicken muscle extract, EzStandard II,
EzStandard LMW, EzProtein Ladder
Gel stain : EzStain AQua
Photographic equipment : LuminoGraph I CMOS
filters : ND-0.1 (optional)
light source : FLAT-Viewer (optional)
Exposure time : 300 ms